Isolation and chromosomal localization of a novel nonerythroid ankyrin gene

Genomics. 1991 Aug;10(4):858-66. doi: 10.1016/0888-7543(91)90173-c.


Immunoreactive isoforms of erythrocyte ankyrin have been shown to be present in a variety of nonerythroid tissues. Isolation of the genes that encode these isoforms will clarify their relationship to erythrocyte ankyrin. Using an erythrocyte ankyrin cDNA clone as a hybridization probe, we screened a human genomic library and isolated a clone that hybridizes with the probe at low stringency but not at high stringency. Partial nucleotide sequence of the clone revealed the presence of a 99-bp segment that is homologous to an exon of the erythrocyte ankyrin gene. Northern analysis showed that a labeled fragment of the clone hybridized to a 7-kb message in RNA of fetal brain but not of erythroid cells, suggesting that this clone is part of a novel gene that is expressed predominantly in nonerythroid tissue. Comparison of the sequence of the genomic clone with that of a recently isolated cDNA clone for brain ankyrin (Otto et al., 1989) showed identity of 96 of 99 bp between the putative exon and a segment of the cDNA clone (V. Bennett, personal communication, 1991), suggesting that the genomic clone is part of a gene for nonerythroid ankyrin, which we have designated ANK2. By analysis of somatic cell hybrids and fluorescence in situ hybridization, we assigned ANK2 to human chromosome 4 at a position equivalent to bands 4q25-q27.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Ankyrins
  • Base Sequence
  • Blood Proteins / genetics*
  • Cell Line
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 4*
  • Cloning, Molecular
  • Cricetinae
  • DNA / genetics
  • DNA / isolation & purification
  • Erythrocytes / physiology*
  • Exons
  • Genes
  • Genomic Library
  • Humans
  • Hybrid Cells / physiology
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Organ Specificity
  • Restriction Mapping


  • Ankyrins
  • Blood Proteins
  • Membrane Proteins
  • DNA

Associated data

  • GENBANK/M37123