Kinetics of asthma- and allergy-associated immune response gene expression in peripheral blood mononuclear cells from vaccinated infants after in vitro re-stimulation with vaccine antigen

Vaccine. 2008 Mar 25;26(14):1725-30. doi: 10.1016/j.vaccine.2008.01.041. Epub 2008 Feb 13.

Abstract

The global expression of immune response genes in infants after vaccination and their role in asthma and allergy is not clearly understood. Pharmacogenomics is ideally suited to study the involved cellular responses, since the expression of thousands of genes can be assessed simultaneously. Here, array technology was used to assess the expression kinetics of immune response genes with association to asthma and allergy in peripheral blood mononuclear cells (PBMC) of five healthy infants after vaccination with Infanrix-Polio+Hib. At 12h after in vitro re-stimulation of the PBMC with pertussis toxin (PT) antigen, 14 immune response pathways, 33 allergy-related and 66 asthma-related genes were found activated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / immunology*
  • Asthma / genetics*
  • Asthma / immunology*
  • Computational Biology
  • Gene Expression / immunology*
  • Haemophilus Vaccines / immunology
  • Humans
  • Hypersensitivity / genetics*
  • Hypersensitivity / immunology*
  • Immunity / genetics*
  • Infant, Newborn
  • Lymphocytes / immunology
  • Monocytes / immunology*
  • Multigene Family / immunology
  • Oligonucleotide Array Sequence Analysis
  • Pertussis Toxin / pharmacology
  • Poliovirus Vaccines / immunology
  • RNA / biosynthesis
  • RNA / genetics
  • Vaccination
  • Vaccines / immunology*

Substances

  • Antigens
  • Haemophilus Vaccines
  • Poliovirus Vaccines
  • Vaccines
  • RNA
  • Pertussis Toxin