Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana

Biotechnol Lett. 2008 Jul;30(7):1289-94. doi: 10.1007/s10529-008-9685-6. Epub 2008 Mar 13.

Abstract

Mitochondrial F(1)F(0)-ATPase is a key enzyme in plant metabolism, providing cells with ATP that uses the transmembrane electrochemical proton gradient to drive synthesis of ATP. A 6 kDa protein (At3g46430) has been previously purified from Arabidopsis thaliana mitochondrial F(1)F(0)-ATPase. In this study, the gene (AtMtATP6; GenBank accession no. AK117680) encoding this protein was isolated from Arabidopsis and characterized. Northern blot analyses showed that the expression of AtMtATP6 gene in Arabidopsis suspension-cultured cells was induced by several abiotic stresses from salts, drought, and cold. Over-expression of AtMtATP6 gene in transgenic yeast and Arabidopsis plants increased the resistance to salts, drought, oxidative and cold stresses. Taken together, our data raise the possibility that induction of the F(1)F(0)-ATPase plays a role in stress tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / biosynthesis*
  • Arabidopsis Proteins / genetics
  • Cold Temperature
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Mitochondrial Proteins / biosynthesis*
  • Mitochondrial Proteins / genetics
  • Peroxins
  • Proton-Translocating ATPases / genetics
  • Proton-Translocating ATPases / metabolism*
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Salts / pharmacology

Substances

  • Arabidopsis Proteins
  • Membrane Proteins
  • Mitochondrial Proteins
  • Peroxins
  • Recombinant Proteins
  • Salts
  • peroxin IIb, Arabidopsis
  • Proton-Translocating ATPases