Effect of berberine on PPARalpha/delta/gamma expression in type 2 diabetic rat retinae

Yao Xue Xue Bao. 2007 Dec;42(12):1243-9.


Retinopathy is a major cause of morbidity in diabetes and remains the primary cause of new blindness. Therefore, it is necessary to find new drug to treat diabetic retinopathy. Type 2 diabetes mellitus (T2DM) rats were induced by injection (ip) with streptozotocin (STZ) 35 mg x kg(-1) and fed with a high-carbohydrate/high-fat diet 2 weeks later. From week 17 to 32, diabetic rats were given different doses of berberine 75, 150, and 300 mg x kg(-1), fenofibrate 100 mg x kg(-1) and rosiglitazone 4 mg x kg(-1), separately. Retinal structure was observed with hematoxylin-eosin staining and peroxisome proliferator-activated receptors (PPARs) alpha/delta/gamma protein expressions were detected by immunohistochemistry. The retina of control rats was thicker than that of other groups, 16 weeks treatment with berberine (150 and 300 mg x kg(-1)) and rosiglitazone 4 mg x kg(-1) thickened the diabetic retina, but no difference existed in retinal structure among groups. Both berberine (150 and 300 mg x kg(-1)) and rosiglitazone 4 mg x kg(-1) significantly decreased PPARy expression in diabetic retina; while berberine (150 and 300 mg x kg(-1)) and fenofibrate 100 mg x kg(-1) obviously increased both PPARalpha and PPARdelta expressions in diabetic retina. Berberine modulates PPARalpha/delta/gamma protein levels in diabetic retina which may contribute to ameliorate retinopathy complication induced by STZ and a high-carbohydrate/high-fat diet. It is expected that berberine might be a more beneficial drug to treat diabetic retinal complication comparing with fenofibrate and rosiglitazone.

MeSH terms

  • Animals
  • Berberine / pharmacology*
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetes Mellitus, Type 2 / metabolism
  • Diabetic Retinopathy / metabolism*
  • Fenofibrate / pharmacology
  • Hypoglycemic Agents / pharmacology
  • Male
  • PPAR alpha / metabolism*
  • PPAR delta / metabolism*
  • PPAR gamma / metabolism*
  • Rats
  • Rats, Wistar
  • Retina / metabolism
  • Retina / pathology
  • Rosiglitazone
  • Thiazolidinediones / pharmacology


  • Hypoglycemic Agents
  • PPAR alpha
  • PPAR delta
  • PPAR gamma
  • Thiazolidinediones
  • Rosiglitazone
  • Berberine
  • Fenofibrate