Determination of voriconazole in human plasma and saliva using high-performance liquid chromatography with fluorescence detection

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):74-80. doi: 10.1016/j.jchromb.2008.02.009. Epub 2008 Mar 10.

Abstract

Voriconazole is a widely used triazole antifungal agent with a broad spectrum including Aspergillus species. A simple, sensitive and selective high-performance liquid chromatography method for the determination of voriconazole in human plasma and saliva was developed. Drug and internal standard (UK-115 794) were extracted from alkaline plasma and saliva with n-hexane-ethyl acetate (3:1, v/v) and analyzed on a Luna C 18 column with fluorimetric detection set at excitation and emission wavelengths of 254 and 372 nm, respectively. The calibration curve was linear through the range of 0.1-10 microg/ml using a 0.3 ml sample volume. The intra- and inter-day precisions were all below 6.1% for plasma and below 9.1% for saliva. Accuracies ranged from 94 to 109% for both matrices. Mean recovery was 86+/-4% for voriconazole. The method showed acceptable values for precision, recovery and sensitivity and is well suited for routine analysis work and for pharmacokinetic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / analysis*
  • Antifungal Agents / blood
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Pyrimidines / analysis*
  • Pyrimidines / blood
  • Reference Standards
  • Reproducibility of Results
  • Saliva / chemistry*
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*
  • Triazoles / analysis*
  • Triazoles / blood
  • Voriconazole

Substances

  • Antifungal Agents
  • Pyrimidines
  • Triazoles
  • Voriconazole