Transcription factor substitution during the evolution of fungal ribosome regulation

Mol Cell. 2008 Mar 14;29(5):552-62. doi: 10.1016/j.molcel.2008.02.006.

Abstract

Coordinated ribosomal protein (RP) gene expression is crucial for cellular viability, but the transcriptional network controlling this regulon has only been well characterized in the yeast Saccharomyces cerevisiae. We have used whole-genome transcriptional and location profiling to establish that, in Candida albicans, the RP regulon is controlled by the Myb domain protein Tbf1 working in conjunction with Cbf1. These two factors bind both the promoters of RP genes and the rDNA locus; Tbf1 activates transcription at these loci and is essential. Orthologs of Tbf1 bind TTAGGG telomeric repeats in most eukaryotes, and TTAGGG cis-elements are present upstream of RP genes in plants and fungi, suggesting that Tbf1 was involved in both functions in ancestral eukaryotes. In all Hemiascomycetes, Rap1 substituted Tbf1 at telomeres and, in the S. cerevisiae lineage, this substitution also occurred independently at RP genes, illustrating the extreme adaptability and flexibility of transcriptional regulatory networks.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Candida albicans / genetics
  • Candida albicans / metabolism
  • Computational Biology
  • DNA, Ribosomal / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Evolution, Molecular*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal*
  • Genome, Fungal
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Regulon
  • Ribosomes / genetics
  • Ribosomes / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Telomere / metabolism
  • Telomere-Binding Proteins / genetics
  • Telomere-Binding Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • CBF1 protein, Candida albicans
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Fungal Proteins
  • RAP1 protein, Candida albicans
  • RAP1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TBF1 protein, S cerevisiae
  • Telomere-Binding Proteins
  • Transcription Factors