Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos

EMBO Rep. 2008 May;9(5):465-71. doi: 10.1038/embor.2008.34. Epub 2008 Mar 14.


By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila / embryology*
  • Drosophila / genetics
  • Drosophila Proteins / physiology
  • Embryo, Nonmammalian
  • GATA Transcription Factors / physiology
  • Gene Expression*
  • Green Fluorescent Proteins / metabolism
  • Hemocytes / physiology
  • Homozygote
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Macrophages / physiology*
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Up-Regulation
  • Wounds and Injuries / etiology
  • Wounds and Injuries / genetics


  • Drosophila Proteins
  • GADD45 protein
  • GATA Transcription Factors
  • Intracellular Signaling Peptides and Proteins
  • srp protein, Drosophila
  • Green Fluorescent Proteins