Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

Oncogene. 2008 Jul 10;27(30):4242-8. doi: 10.1038/onc.2008.56. Epub 2008 Mar 17.

Abstract

The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cell Transformation, Neoplastic / genetics*
  • Cell Transformation, Neoplastic / radiation effects*
  • Cleavage Stage, Ovum
  • DNA-Binding Proteins / administration & dosage
  • DNA-Binding Proteins / genetics
  • Embryo, Nonmammalian
  • Gene Transfer Techniques*
  • Genes, bcl-2
  • Genes, myc
  • Genes, p53
  • Green Fluorescent Proteins / administration & dosage
  • Green Fluorescent Proteins / genetics
  • Luminescent Proteins / administration & dosage
  • Luminescent Proteins / genetics
  • Microinjections / methods*
  • Mutant Proteins / genetics
  • Neoplasms, Radiation-Induced / genetics*
  • Nuclear Proteins / administration & dosage
  • Nuclear Proteins / genetics
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / genetics
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins p21(ras)
  • Transgenes
  • Zebrafish / embryology
  • ras Proteins / genetics

Substances

  • DNA-Binding Proteins
  • KRAS protein, human
  • Luminescent Proteins
  • Mutant Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RAG2 protein, human
  • red fluorescent protein
  • Green Fluorescent Proteins
  • Proto-Oncogene Proteins p21(ras)
  • ras Proteins