A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor delta-chain-encoding gene

N van der Lugt; E R Maandag; H te Riele; P W Laird; A Berns

doi:10.1016/0378-1119(91)90161-4

A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor delta-chain-encoding gene

Gene. 1991 Sep 15;105(2):263-7. doi: 10.1016/0378-1119(91)90161-4.

Authors

N van der Lugt¹, E R Maandag, H te Riele, P W Laird, A Berns

Affiliation

¹ Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.

PMID: 1834524
DOI: 10.1016/0378-1119(91)90161-4

Abstract

We have constructed a hypoxanthine phosphoribosyl transferase-selectable marker (hprt) under the control of the phosphoglycerate kinase (pgk) promoter. This construct permits cell growth in hypoxanthine/aminopterin/thymidine media and confers 6-thioguanine sensitivity upon mouse Hprt- embryonic stem cells, allowing either positive or negative selection in gene-targeting experiments. We have successfully targeted the gene encoding the T-cell receptor delta-chain using the pgk::hprt fusion for counterselection.

MeSH terms

Animals
Blotting, Southern
Genetic Markers*
Hypoxanthine Phosphoribosyltransferase / genetics*
Mice
Phosphoglycerate Kinase / genetics*
Plasmids
Receptors, Antigen, T-Cell, gamma-delta / genetics*
Recombinant Fusion Proteins / genetics
Stem Cells

Substances

Genetic Markers
Receptors, Antigen, T-Cell, gamma-delta
Recombinant Fusion Proteins
Hypoxanthine Phosphoribosyltransferase
Phosphoglycerate Kinase