Abstract
Live attenuated recombinant measles viruses (rMV) expressing a codon-optimised spike glycoprotein (S) or nucleocapsid protein (N) of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) were generated (rMV-S and rMV-N). Both recombinant viruses stably expressed the corresponding SARS-CoV proteins, grew to similar end titres as the parental strain and induced high antibody titres against MV and the vectored SARS-CoV antigens (S and N) in transgenic mice susceptible to measles infection. The antibodies induced by rMV-S had a high neutralising effect on SARS-CoV as well as on MV. Moreover, significant N-specific cellular immune responses were measured by IFN-gamma ELISPOT assays. The pre-existence of anti-MV antibodies induced by the initial immunisation dose did not inhibit boost of anti-S and anti-N antibodies. Immunisations comprising a mixture of rMV-S and rMV-N induced immune responses similar in magnitude to that of vaccine components administered separately. These data support the suitability of MV as a bivalent candidate vaccine vector against MV and emerging viruses such as SARS-CoV.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Animals, Genetically Modified
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Genetic Vectors / chemistry
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Measles Vaccine / administration & dosage
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Measles Vaccine / genetics
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Measles Vaccine / immunology
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Measles virus / metabolism
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Measles virus / physiology*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology*
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Mice
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Neutralization Tests
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Nucleocapsid Proteins / genetics
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Severe Acute Respiratory Syndrome / immunology
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Severe Acute Respiratory Syndrome / prevention & control*
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Severe acute respiratory syndrome-related coronavirus / genetics*
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Severe acute respiratory syndrome-related coronavirus / immunology
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Severe acute respiratory syndrome-related coronavirus / metabolism
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Spike Glycoprotein, Coronavirus
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology
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Viral Envelope Proteins / immunology*
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Viral Vaccines / administration & dosage*
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Viral Vaccines / chemistry
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Viral Vaccines / genetics
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Viral Vaccines / immunology
Substances
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Measles Vaccine
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Membrane Glycoproteins
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Nucleocapsid Proteins
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Spike Glycoprotein, Coronavirus
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Vaccines
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spike glycoprotein, SARS-CoV