Comparing whole genomes using DNA microarrays

Nat Rev Genet. 2008 Apr;9(4):291-302. doi: 10.1038/nrg2335.

Abstract

The rapid accumulation of complete genomic sequences offers the opportunity to carry out an analysis of inter- and intra-individual genome variation within a species on a routine basis. Sequencing whole genomes requires resources that are currently beyond those of a single laboratory and therefore it is not a practical approach for resequencing hundreds of individual genomes. DNA microarrays present an alternative way to study differences between closely related genomes. Advances in microarray-based approaches have enabled the main forms of genomic variation (amplifications, deletions, insertions, rearrangements and base-pair changes) to be detected using techniques that are readily performed in individual laboratories using simple experimental approaches.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Chromosome Mapping
  • Gene Dosage
  • Genetic Variation*
  • Genome*
  • Genomics / methods
  • Genomics / statistics & numerical data
  • Humans
  • Oligonucleotide Array Sequence Analysis* / methods
  • Oligonucleotide Array Sequence Analysis* / statistics & numerical data
  • Polymorphism, Single Nucleotide