A multiplex TaqMan PCR assay for the detection of measles and rubella virus

J Virol Methods. 2008 May;149(2):246-50. doi: 10.1016/j.jviromet.2008.01.032. Epub 2008 Mar 18.


Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers / genetics
  • Measles / diagnosis*
  • Measles virus / genetics
  • Measles virus / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • RNA, Viral / genetics
  • Rubella / diagnosis*
  • Rubella virus / genetics
  • Rubella virus / isolation & purification*
  • Sequence Alignment


  • DNA Primers
  • RNA, Viral