Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays

Cancer Lett. 2008 Jun 28;265(1):98-106. doi: 10.1016/j.canlet.2008.02.006. Epub 2008 Mar 18.

Abstract

Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Antibodies
  • Antigens, CD / immunology*
  • B-Lymphocytes / immunology*
  • Child
  • Child, Preschool
  • Gene Expression Profiling
  • Humans
  • Immunophenotyping
  • Lymphocyte Subsets / immunology
  • Lymphoma, B-Cell / immunology*
  • Oligonucleotide Array Sequence Analysis
  • Principal Component Analysis
  • Protein Array Analysis
  • Receptors, Antigen, B-Cell / immunology*

Substances

  • Antibodies
  • Antigens, CD
  • Receptors, Antigen, B-Cell