The role of the OOP antisense RNA in coliphage lambda development

Mol Microbiol. 1991 May;5(5):1265-72. doi: 10.1111/j.1365-2958.1991.tb01900.x.


We have made a derivative of bacteriophage lambda that makes no OOP antisense RNA. The mutant phage carries a point mutation that inactivates the OOP promoter, po. The phages lambda + and lambda po- have identical plaque morphologies, one-step growth curves, and frequencies of lysogenization of a sensitive host. OOP RNA synthesis is weakly repressed by the Escherichia coli LexA protein. Consonant with this inducibility of OOP RNA synthesis by ultraviolet light, we find a two-fold greater phage burst following ultraviolet induction of a lambda + than of a lambda po- prophage. In lambda + infections, OOP RNA causes two cleavage events in cll mRNA: one is in the 3'-end of the coding region, and the second is in the intercistronic region between the cll and O genes. The cll gene fragments are subject to additional hydrolytic events, and cll mRNA levels are several-fold lower in lambda + than in lambda po- infections late in the infection cycle. However, O mRNA levels are almost unaffected by the po- mutation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism
  • Bacteriophage lambda / genetics*
  • Bacteriophage lambda / growth & development
  • Base Sequence
  • Endoribonucleases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins*
  • Gene Expression Regulation, Viral* / radiation effects
  • Genes, Viral*
  • Lysogeny
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA Processing, Post-Transcriptional*
  • RNA, Antisense / physiology*
  • RNA, Messenger / metabolism
  • RNA, Viral / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / metabolism
  • Ribonuclease III
  • Serine Endopeptidases*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Ultraviolet Rays
  • Viral Proteins
  • Viral Structural Proteins / genetics*
  • Virus Activation


  • Bacterial Proteins
  • Escherichia coli Proteins
  • LexA protein, Bacteria
  • RNA, Antisense
  • RNA, Messenger
  • RNA, Viral
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Transcription Factors
  • Viral Proteins
  • Viral Structural Proteins
  • cII protein, bacteriophage lambda
  • Endoribonucleases
  • Ribonuclease III
  • ribonuclease III, E coli
  • Serine Endopeptidases