Controlled release of insulin-like growth factor-1 and bone marrow stromal cell function of bone-like mineral layer-coated poly(lactic-co-glycolic acid) scaffolds

Abstract

Controlled release of growth factors or drugs provides great therapeutic advantages for bone defects which do not heal with normal therapeutic treatments. We have accelerated the deposition of bone-like mineral (BLM) on the surface of three-dimensional (3D) poly(lactic-co-glycolic acid) (PLGA) porous scaffolds to 36-48 h by modifying the biomimetic process parameters and applying surface treatments onto PLGA scaffolds. We used simulated body fluid containing insulin-like growth factor-1 (IGF-1; 1 microg/ml) to mineralize the PLGA scaffolds for 48 h. IGF-1 was co-precipitated with mineral on the surface of the PLGA scaffolds. IGF-1-incorporated mineralized scaffolds demonstrated slow controlled release over a 30 day period when they were incubated in phosphate-buffered saline (PBS) at 37 degrees C. Bone marrow stromal cell (BMSC) function on three different types of scaffolds, such as control (non-mineralized) scaffolds, mineralized scaffolds, and IGF-1-incorporated mineralized scaffolds was also investigated. BMSC attachment and proliferation was enhanced for IGF-1-incorporated mineralized scaffolds compared with controls during the culture period. BMSC differentiation was not changed during the culture period among the three groups of scaffolds, as assessed by alkaline phosphatase activity and osteocalcin assay. According to findings from this study, BLM has great potential to be used as a carrier for biological molecules for localized release applications as well as bone tissue-engineering applications.