Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Biotechniques. 2008 Mar;44(3):413-6. doi: 10.2144/000112706.


The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

MeSH terms

  • Animals
  • Blastocyst / cytology*
  • Blastocyst / physiology*
  • Cells, Cultured
  • Embryo, Mammalian / cytology*
  • Embryo, Mammalian / physiology*
  • Genetic Engineering / methods*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout / embryology
  • Mice, Knockout / genetics*
  • Mice, Knockout / growth & development*