Expression of claudin-2 in the multistage process of gastric carcinogenesis

Histol Histopathol. 2008 Jun;23(6):673-82. doi: 10.14670/HH-23.673.

Abstract

Objective: Claudin-2 is an important component of the tight junction (TJ) of epithelial cells, and its protein expression between many tumours is significantly different. It is unclear if Claudin-2 overexpression is a trigger or a consequence during carcinogenesis. The multistage tissues of gastric carcinogenesis provides us a valuable model for determining tumourigenicity.

Methods: This paper investigated, for the first time, claudin-2 expression in the pathological paraffin tissues of sinus ventriculi from gastroscopic biopsy. To determine claudin-2 expression in gastric carcinogenesis by immunochemical ABC technique.

Results: Altogether, 108 chronic superficial gastritis, 55 chronic atrophic gastritis, 109 intestinal-type metaplasia, 93 dysplasia and 52 gastric intestinal-type adenocarcinoma samples were analyzed. Results indicated that the percentage of claudin-2-positive cases was 0% for chronic superficial gastritis (0/108), 0% for chronic atrophic gastritis (0/55), 0% for intestinal-type metaplasia (0/109), 35.87% for dysplasia (33/92), and 73.47% for gastric intestinal-type adenocarcinoma (36/49) respectively, primarily in the cell membrane, and gradually increased in the multistage process of gastric carcinogenesis (P<0.001).

Conclusions: This suggests that claudin-2 protein overexpression may be closely correlated to gastric carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism
  • Claudins
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Gastric Mucosa / metabolism*
  • Gastric Mucosa / pathology
  • Gastritis, Atrophic / metabolism
  • Gastritis, Atrophic / pathology
  • Humans
  • Hyperplasia / metabolism
  • Hyperplasia / pathology
  • Immunoenzyme Techniques
  • Male
  • Membrane Proteins / metabolism*
  • Metaplasia / metabolism
  • Metaplasia / pathology
  • Middle Aged
  • Precancerous Conditions / metabolism*
  • Precancerous Conditions / pathology
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • CLDN2 protein, human
  • Claudins
  • Membrane Proteins