Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome

Fertil Steril. 2009 Apr;91(4 Suppl):1307-10. doi: 10.1016/j.fertnstert.2008.01.078. Epub 2008 Mar 25.


Sperm analysis following World Health Organization guidelines is unable to explain the molecular causes of male infertility when basic sperm parameters are within a normal range and women do not present gynecologic pathology. Consequently, there is a need for accurate diagnostic tools in this area, and microarray technology emerges as promising. We present, for the first time, preliminary results of a comparison of sperm mRNA expression profiles between fertile and infertile men with normal semen parameters, discovering profound discrepancies between groups, with potential diagnostic and therapeutic possibilities.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / metabolism
  • Apoptosis Regulatory Proteins / metabolism
  • DNA / genetics
  • Fertility / genetics*
  • Gene Expression Profiling*
  • Humans
  • Infertility, Male / genetics*
  • Male
  • Oligonucleotide Array Sequence Analysis*
  • RNA, Messenger / metabolism
  • Semen / cytology
  • Semen / metabolism
  • Spermatozoa / abnormalities
  • Spermatozoa / metabolism*
  • Trypsin
  • Trypsinogen / metabolism
  • gamma-Glutamyltransferase / metabolism


  • Antigens, Neoplasm
  • Apoptosis Regulatory Proteins
  • CAB39L protein, human
  • RNA, Messenger
  • Trypsinogen
  • DNA
  • gamma-Glutamyltransferase
  • PRSS1 protein, human
  • Trypsin