Reciprocal hybrid joints demonstrate successive V-J rearrangements on the same chromosome in the human TCR gamma locus

Int Immunol. 1991 Oct;3(10):973-82. doi: 10.1093/intimm/3.10.973.


Novel variable (V)--joining (J) gene rearrangements are described in the human T cell receptor gamma locus, in which, on the one hand, the V3 variable gene is joined to the heptamer--nonamer recombination signals of the J1 segment and, on the other hand, the J1 segment is joined to the V3 recombination signals through head-to-head fusion. These recombination products, or hybrid joints, have been originated through an inversion of 47 kb DNA. Interestingly the inverted DNA stretch contains a normal V9-J9 rearrangement. These findings are the first direct demonstration that successive rearrangements occur, on the same chromosome, in the human T cell receptor gamma locus, and suggest that the chronology of the joining events plays a role in the ontogeny of T cells and their differentiation in gamma/delta + and alpha/beta + lineages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence*
  • Chromosome Inversion
  • Clone Cells
  • DNA Nucleotidyltransferases / metabolism
  • Gene Rearrangement, T-Lymphocyte / genetics*
  • Gene Rearrangement, T-Lymphocyte / immunology
  • Humans
  • Integrases*
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell, gamma-delta / genetics*
  • Recombinases
  • Recombination, Genetic
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Severe Combined Immunodeficiency / genetics
  • Severe Combined Immunodeficiency / immunology


  • Receptors, Antigen, T-Cell, gamma-delta
  • Recombinases
  • DNA Nucleotidyltransferases
  • Integrases
  • integron integrase IntI1