SNP-specific array-based allele-specific expression analysis

Genome Res. 2008 May;18(5):771-9. doi: 10.1101/gr.073254.107. Epub 2008 Mar 27.

Abstract

We have developed an optimized array-based approach for customizable allele-specific gene expression (ASE) analysis. The central features of the approach are the ability to select SNPs at will for detection, and the absence of need to PCR amplify the target. A surprisingly long probe length (39-49 nt) was needed for allelic discrimination. Reconstitution experiments demonstrate linearity of ASE over a broad range. Using this approach, we have discovered at least two novel imprinted genes, NLRP2, which encodes a member of the inflammasome, and OSBPL1A, which encodes a presumed oxysterol-binding protein, were both preferentially expressed from the maternal allele. In contrast, ERAP2, which encodes an aminopeptidase, did not show preferential parent-of-origin expression, but rather, cis-acting nonimprinted differential allelic control. The approach is scalable to the whole genome and can be used for discovery of functional epigenetic modifications in patient samples.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Alleles*
  • Aminopeptidases / genetics
  • Carrier Proteins / genetics
  • Cell Line
  • Genomic Imprinting
  • Heterozygote
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymorphism, Single Nucleotide*
  • Receptors, Steroid
  • Reproducibility of Results

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • NLRP2 protein, human
  • Receptors, Steroid
  • oxysterol binding protein
  • Aminopeptidases
  • ERAP2 protein, human