ENU mutagenesis in mice

Methods Mol Biol. 2008;415:1-16. doi: 10.1007/978-1-59745-570-1_1.


Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Mapping
  • Disease Susceptibility
  • Ethylnitrosourea / toxicity*
  • Female
  • Genome
  • Herpesviridae Infections / virology
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Biology / methods*
  • Muromegalovirus / drug effects
  • Mutagenesis / drug effects*
  • Pedigree
  • Phenotype
  • Signal Transduction / drug effects
  • Toll-Like Receptors / metabolism


  • Toll-Like Receptors
  • Ethylnitrosourea