Detection of siRNAs and miRNAs

Methods Mol Biol. 2008;451:217-27. doi: 10.1007/978-1-59745-102-4_15.

Abstract

Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel / methods
  • Gene Silencing*
  • In Situ Hybridization / methods
  • MicroRNAs / analysis
  • MicroRNAs / genetics*
  • Oligonucleotide Probes
  • Plant Viruses / genetics*
  • Plants / virology
  • RNA, Small Interfering / analysis
  • RNA, Small Interfering / genetics*
  • RNA, Viral / analysis
  • RNA, Viral / genetics

Substances

  • MicroRNAs
  • Oligonucleotide Probes
  • RNA, Small Interfering
  • RNA, Viral