Pregnancy after trophectoderm biopsy of frozen-thawed blastocyst

Fertil Steril. 2009 May;91(5):1938-40. doi: 10.1016/j.fertnstert.2008.02.132. Epub 2008 Apr 18.


Objective: To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.

Design: Techniques and instrumentation.

Setting: A University Medical Center.

Patient(s): Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.

Intervention(s): Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.

Main outcome measurement(s): Serum betahCG and transvaginal ultrasound.

Result(s): Positive betahCG and ongoing pregnancy.

Conclusion(s): Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Biopsy
  • Blastocyst / physiology*
  • Chorionic Gonadotropin, beta Subunit, Human / blood
  • Cleavage Stage, Ovum / pathology
  • Embryo Transfer*
  • Female
  • Freezing
  • Humans
  • In Situ Hybridization, Fluorescence
  • Pregnancy
  • Preimplantation Diagnosis / methods*
  • Trophoblasts / cytology


  • Chorionic Gonadotropin, beta Subunit, Human