DNA microarray-based genotyping of methicillin-resistant Staphylococcus aureus strains from Eastern Saxony

Clin Microbiol Infect. 2008 Jun;14(6):534-45. doi: 10.1111/j.1469-0691.2008.01986.x. Epub 2008 Mar 27.


A diagnostic microarray was used to characterise a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates from hospitals in the German region of Eastern Saxony. The most abundant epidemic MRSA (EMRSA) strains were ST5-MRSA II (Rhine-Hesse EMRSA, EMRSA-3), CC5/ST228-MRSA I (South German EMRSA), ST22-MRSA IV (Barnim EMRSA, EMRSA-15) and ST45-MRSA IV (Berlin EMRSA). Other strains were found only as sporadic isolates or in minor outbreaks. These strains included ST1-MRSA IV, ST8-MRSA IV (Hannover EMRSA and others), clonal group 5 strains carrying SCCmec type IV elements (Paediatric clone), ST45-MRSA V, CC8/ST239-MRSA III and ST398-MRSA V. Panton-Valentine leukocidin-positive MRSA isolates were still very rare. The predominant strain was ST80-MRSA IV, although increasing numbers of different strains have recently been detected (ST8-MRSA IV, ST30-MRSA IV and ST59-MRSA V). For more common MRSA strains, it was possible to detect variants that differed mainly in the carriage of additional resistance determinants and certain virulence-associated genes. Detection of such variants can sometimes allow epidemic strains to be resolved beyond spa types to a hospital-specific level, which is of significant value for epidemiological purposes.

MeSH terms

  • Bacterial Typing Techniques
  • Cross Infection / epidemiology
  • DNA, Bacterial / analysis
  • Disease Outbreaks*
  • Genotype
  • Germany / epidemiology
  • Humans
  • Methicillin Resistance*
  • Oligonucleotide Array Sequence Analysis*
  • Staphylococcal Infections / epidemiology*
  • Staphylococcus aureus / genetics*


  • DNA, Bacterial