Analysis of Rab GTPase and GTPase-activating protein function at primary cilia

Methods Enzymol. 2008:439:353-64. doi: 10.1016/S0076-6879(07)00426-0.

Abstract

Primary cilia are sensory structures on the cell surface whose formation requires tight integration of microtubules and polarized membrane trafficking events. Rabs are GTP-binding proteins of the Ras superfamily that control directed membrane trafficking by regulating membrane-membrane and membrane-cytoskeleton interactions. This chapter describes cell biological and biochemical methods for the analysis of Rab function and Rab GTPase-activating proteins during primary cilia formation.

MeSH terms

  • Cell Cycle Proteins
  • Cell Line
  • Cilia / metabolism*
  • Cloning, Molecular
  • GTPase-Activating Proteins / analysis*
  • Heat-Shock Proteins / analysis
  • Heat-Shock Proteins / physiology
  • Humans
  • Microtubule-Associated Proteins / analysis
  • Nuclear Proteins / isolation & purification
  • rab GTP-Binding Proteins / analysis*
  • rab GTP-Binding Proteins / physiology

Substances

  • Cell Cycle Proteins
  • EVI5 protein, human
  • GTPase-Activating Proteins
  • Heat-Shock Proteins
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • ODF2 protein, human
  • RABGAP1 protein, human
  • RAB8A protein, human
  • rab GTP-Binding Proteins