Coenzyme F420-dependent sulfite reductase-enabled sulfite detoxification and use of sulfite as a sole sulfur source by Methanococcus maripaludis

Appl Environ Microbiol. 2008 Jun;74(11):3591-5. doi: 10.1128/AEM.00098-08. Epub 2008 Mar 31.

Abstract

Coenzyme F(420)-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, was expressed with activity in Methanococcus maripaludis, a sulfite-sensitive methanogen. The recombinant organism reduced sulfite to sulfide and grew with sulfite as the sole sulfur source, indicating that Fsr is a sulfite detoxification and assimilation enzyme for methanogens and that M. maripaludis synthesizes siroheme.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cloning, Molecular
  • Gene Expression
  • Methanococcales / enzymology*
  • Methanococcales / genetics
  • Methanococcales / growth & development
  • Methanococcales / metabolism*
  • Oxidation-Reduction
  • Oxidoreductases Acting on Sulfur Group Donors / genetics
  • Oxidoreductases Acting on Sulfur Group Donors / metabolism*
  • Riboflavin / analogs & derivatives*
  • Riboflavin / metabolism
  • Sulfides / metabolism
  • Sulfites / metabolism*
  • Sulfur / metabolism

Substances

  • Sulfides
  • Sulfites
  • coenzyme F420
  • Sulfur
  • Oxidoreductases Acting on Sulfur Group Donors
  • Riboflavin