Proliferation and interleukin 5 production by CD8hi CD57+ T cells

Eur J Immunol. 2008 Apr;38(4):995-1000. doi: 10.1002/eji.200737687.


CD8hi CD57+ T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation-induced cell death. In contrast, we demonstrate here that CD8hi CD57+ T cells are capable of rapid expansion using multiple techniques including [(3)H]thymidine uptake, flow cytometric bead-based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation-induced expansion and increased 7-amino-actinomycin D uptake by CD8hi CD57+ T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8hi CD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin 5. These data indicate that CD8hi CD57+ T cells should not be considered as "end-stage" effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN-gamma responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • CD57 Antigens / immunology*
  • CD8 Antigens / metabolism*
  • CD8-Positive T-Lymphocytes / cytology*
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism*
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Dactinomycin / analogs & derivatives
  • Dactinomycin / pharmacology
  • Fluoresceins / pharmacology
  • Humans
  • Interleukin-5 / biosynthesis*
  • Middle Aged
  • Succinimides / pharmacology


  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • CD57 Antigens
  • CD8 Antigens
  • Fluoresceins
  • Interleukin-5
  • Succinimides
  • Dactinomycin
  • 7-aminoactinomycin D