CRIP homologues maintain apical cytoskeleton to regulate tubule size in C. elegans

Dev Biol. 2008 May 1;317(1):225-33. doi: 10.1016/j.ydbio.2008.02.040. Epub 2008 Mar 4.


Maintenance of the shape and diameter of biological tubules is a critical task in the development and physiology of all metazoan organisms. We have cloned the exc-9 gene of Caenorhabditis elegans, which regulates the diameter of the single-cell excretory canal tubules. exc-9 encodes a homologue of the highly expressed mammalian intestinal LIM-domain protein CRIP, whose function has not previously been determined. A second well-conserved CRIP homologue functions in multiple valves of C. elegans. EXC-9 shows genetic interactions with other EXC proteins, including the EXC-5 guanine exchange factor that regulates CDC-42 activity. EXC-9 and its nematode homologue act in polarized epithelial cells that must maintain great flexibility at their apical surface; our results suggest that CRIPs function to maintain cytoskeletal flexibility at the apical surface.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / chemistry
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism
  • Cytoskeleton / chemistry
  • Cytoskeleton / metabolism
  • Gene Expression Regulation
  • Mammals
  • Metalloproteins / chemistry
  • Metalloproteins / genetics
  • Metalloproteins / metabolism*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Sequence Alignment


  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Exc-9 protein, C elegans
  • Metalloproteins
  • VALV-1 protein, C elegans