Is the Sequenced Bordetella pertussis strain Tohama I representative of the species?

Valérie Caro; Valérie Bouchez; Nicole Guiso

doi:10.1128/JCM.02484-07

Is the Sequenced Bordetella pertussis strain Tohama I representative of the species?

J Clin Microbiol. 2008 Jun;46(6):2125-8. doi: 10.1128/JCM.02484-07. Epub 2008 Apr 2.

Authors

Valérie Caro¹, Valérie Bouchez, Nicole Guiso

Affiliation

¹ Institut Pasteur, Molecular Prevention and Therapy of Human Diseases Unit, URA-CNRS 3012, National Center of Reference of Whooping Cough and Bordetelloses, Paris, France. vcaro@pasteur.fr

Abstract

Subtractive hybridization was carried out to identify differences between the sequenced genome of Bordetella pertussis Tohama I and those of two recently collected isolates. We identified genetic regions specific to recent isolates, old isolates, and isolates of B. parapertussis and B. bronchiseptica species. We conclude that Tohama I strain is not representative of the B. pertussis species.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bordetella pertussis / classification*
Bordetella pertussis / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism
Electrophoresis, Gel, Pulsed-Field
Genome, Bacterial*
Humans
Nucleic Acid Hybridization / methods*
Reference Standards
Sequence Analysis, DNA*
Species Specificity

Substances

Deoxyribonucleases, Type II Site-Specific
GATC-specific type II deoxyribonucleases
GTAC-specific type II deoxyribonucleases