Gene expression profiles of mouse striatum in control and maneb + paraquat-induced Parkinson's disease phenotype: validation of differentially expressed energy metabolizing transcripts

Mol Biotechnol. 2008 Sep;40(1):59-68. doi: 10.1007/s12033-008-9060-9. Epub 2008 Apr 2.

Abstract

The present study was undertaken to investigate the gene expression patterns of the striatum of control and maneb + paraquat-induced Parkinson's disease (PD) phenotype in mouse to identify the differentially expressed transcripts. The animals were treated with and without maneb (30 mg/kg, i.p.) + paraquat (10 mg/kg, i.p.), twice a week, for 3, 6, and 9 weeks. The RNA was isolated from control and treated mouse striatum and reverse transcribed, and equal quantities of labeled cDNA were mixed and hybridized with mouse 15 k arrays. Comparative transcription patterns showed the time of exposure dependent alteration in the expression of several transcripts associated with various pathways. RT-PCR reconfirmed the differential expression of some energy metabolizing transcripts. The study provides maneb + paraquat-induced differential expression of many transcripts using high-density microarray approach. Few transcripts, which were previously not reported to be associated with neuronal degeneration, were also identified. The results obtained thus suggest that maneb + paraquat induce neurotoxicity in the striatum in a time of exposure dependent manner via multiple pathways and defective energy metabolism could play a critical role.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Blotting, Western
  • Corpus Striatum / drug effects*
  • Corpus Striatum / metabolism
  • Disease Models, Animal
  • Energy Metabolism / genetics*
  • Fungicides, Industrial / pharmacology
  • Gene Expression Profiling*
  • Herbicides / pharmacology
  • Male
  • Maneb / pharmacology*
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Paraquat / pharmacology*
  • Phenotype
  • RNA, Messenger / genetics

Substances

  • Fungicides, Industrial
  • Herbicides
  • RNA, Messenger
  • Maneb
  • Paraquat