Hsp60C is required in follicle as well as germline cells during oogenesis in Drosophila melanogaster

Dev Dyn. 2008 May;237(5):1334-47. doi: 10.1002/dvdy.21524.


Hsp60C gene of Drosophila melanogaster shows a dynamic spatiotemporal expression during oogenesis and seems to contribute bulk of the Hsp60 family proteins in ovarioles. Hsp60 distribution overlaps with that of F-actin-rich membranes/structures in follicle, nurse, and egg cells throughout oogenesis. Skeletal muscle fibers associated with ovarioles and in other parts of the body show patterned location of Hsp60 in A-bands. During stages 11-12, Hsp60 accumulates at junctions of nurse cells and oocyte, where a new microtubule organizing center is known to develop. A recessive hypomorph allele, Hsp60C1 causes complete sterility of the rare surviving homozygous adults. Their egg chambers show very little Hsp60C transcripts or Hsp60 protein. Beginning at stages 6-7, Hsp60C1 chambers show a disorganized follicle cell layer with poor cell adhesion in addition to abnormal organization of F-actin and other cytoskeletal structures in follicle, nurse, and egg cells. Additionally, expression and localizations of Hrb98DE, Squid, and Gurken proteins in nurse cells and oocyte are also severely affected. Hsp60C1 homozygous follicle cell clones in Hsp60C1/+ ovarioles show disruptions in follicle epithelial and cytoskeleton arrangements. Likewise, egg chambers with Hsp60C1 homozygous germline clones in Hsp60C1/+ flies show abnormal oogenesis. Our results provide the first evidence for an essential role of Hsp60C in Drosophila oogenesis, especially in organization and maintenance of cytoskeletal and cell adhesion components.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism
  • Animals
  • Cell Adhesion / physiology
  • Chaperonin 60 / genetics
  • Chaperonin 60 / metabolism*
  • Cytoskeleton / metabolism
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / anatomy & histology
  • Drosophila melanogaster / physiology*
  • Female
  • Gene Expression Regulation, Developmental
  • Germ Cells / cytology
  • Germ Cells / metabolism*
  • Heat-Shock Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins / genetics
  • Heterogeneous-Nuclear Ribonucleoproteins / metabolism
  • Oocytes / cytology
  • Oocytes / physiology
  • Oogenesis / physiology*
  • Ovarian Follicle / cytology
  • Ovarian Follicle / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transforming Growth Factor alpha / genetics
  • Transforming Growth Factor alpha / metabolism


  • Actins
  • Chaperonin 60
  • Drosophila Proteins
  • HRB98DE protein, Drosophila
  • Heat-Shock Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Hsp60C protein, Drosophila
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Transforming Growth Factor alpha
  • grk protein, Drosophila
  • sqd protein, Drosophila