Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins

FEBS Lett. 2008 Apr 30;582(10):1419-24. doi: 10.1016/j.febslet.2008.02.082. Epub 2008 Apr 1.

Abstract

Little is known about the quality control of proteins upon integration in the inner membrane of Escherichia coli. Here, we demonstrate that YidC and FtsH are adjacent to a nascent, truncated membrane protein using in vitro photo cross-linking. YidC plays a critical but poorly understood role in the biogenesis of E. coli inner membrane proteins (IMPs). FtsH functions as a membrane chaperone and protease. Furthermore, we show that FtsH and its modulator proteins HflK and HflC copurify with tagged YidC and, vice versa, that YidC copurifies with tagged FtsH. These results suggest that FtsH and YidC have a linked role in the quality control of IMPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Dependent Proteases / chemistry
  • ATP-Dependent Proteases / isolation & purification
  • ATP-Dependent Proteases / metabolism*
  • Cell Membrane / metabolism
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / isolation & purification
  • Escherichia coli Proteins / metabolism*
  • Membrane Proteins / chemistry
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / isolation & purification
  • Membrane Transport Proteins / metabolism*
  • Peptides / chemistry
  • Peptides / isolation & purification
  • Peptides / metabolism*

Substances

  • Escherichia coli Proteins
  • HflC protein, E coli
  • HflK protein, E coli
  • Membrane Proteins
  • Membrane Transport Proteins
  • Peptides
  • YIDC protein, E coli
  • ATP-Dependent Proteases
  • FtsH protein, E coli