Abstract
A determination method for individual natural vitamin B(6) compounds was developed. The vitamin B(6) compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.
MeSH terms
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Alcohol Oxidoreductases / chemistry*
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Animals
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Capsicum
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Chickens
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Chromatography, High Pressure Liquid / methods
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Food Analysis / methods*
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Garlic
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Glucosides / analysis
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Glucosides / chemistry
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Hydrochloric Acid / chemistry
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Hydrolysis
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Pyridoxic Acid / analogs & derivatives*
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Pyridoxic Acid / analysis
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Pyridoxic Acid / chemical synthesis
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Pyridoxine / analogs & derivatives
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Pyridoxine / analysis
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Pyridoxine / chemistry
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Time Factors
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Transaminases / chemistry*
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Vitamin B 6 / analysis
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Vitamin B 6 / chemistry
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Vitamin B Complex / analysis*
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Vitamin B Complex / chemistry
Substances
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Glucosides
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Vitamin B Complex
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5'-O-(glucopyranosyl)pyridoxine
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4-pyridoxic acid lactone
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Pyridoxic Acid
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Vitamin B 6
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Alcohol Oxidoreductases
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pyridoxal dehydrogenase
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pyridoxine 4-oxidase
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Transaminases
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pyridoxamine-pyruvate transaminase
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Pyridoxine
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Hydrochloric Acid