RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation

Mol Biol Rep. 2009 Apr;36(4):767-74. doi: 10.1007/s11033-008-9243-2. Epub 2008 Apr 4.


Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down method was established. Cell cycle analysis suggests that the cell proliferation decreases after RNAi with mN6A1. The expression plasmid of luciferase was used to detect protein translation, and the results showed that luciferase expression decreased after RNAi with mN6A1, whereas increased after over-expression of mN6A1 or/and eRF1. The binding between mN6A1 and eRF1 was identified by co-immunoprecipitation and pull-down experiments. It might be suggested that mN6A1 participates in protein translation through interaction with eRF1.

MeSH terms

  • Animals
  • Cell Line
  • Cell Proliferation
  • DNA Modification Methylases / genetics
  • DNA Modification Methylases / metabolism*
  • Gene Expression Regulation
  • Gene Knockdown Techniques
  • Genes, Reporter / genetics
  • Humans
  • Methyltransferases / genetics
  • Methyltransferases / metabolism*
  • Mice
  • Molecular Sequence Data
  • Peptide Termination Factors / genetics
  • Peptide Termination Factors / metabolism
  • Protein Binding
  • Protein Biosynthesis / genetics*
  • RNA Interference*
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)


  • Peptide Termination Factors
  • DNA Modification Methylases
  • Methyltransferases
  • PRED28 protein, mouse
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)

Associated data

  • GENBANK/AY456393