Effect of SBD.4A--a defined multicomponent preparation of Angelica sinensis--in periodontal regeneration models

Phytother Res. 2008 Jul;22(7):923-8. doi: 10.1002/ptr.2421.


Periodontitis is a major cause of tooth motility and loss, resulting in destruction of the supporting structures of the tooth, including periodontal ligaments and alveolar bone. Periodontal surgery can slow the progression of the disease, but is costly, invasive, limited by contraindications and technique-sensitive. Recently, non-invasive pharmacological treatments using proteinaceous biologicals have become available. Here, for the first time, the bone-regenerative capabilities of a non-proteinaceous biological--SBD.4A--a novel, stable multicomponent growth factor isolated from a medicinal plant Angelica sinensis are reported. SBD.4A was tested in osteoblast proliferation and differentiation systems, as well as in a fibroblast-secreted hyaluronic acid assay. Furthermore, SBD.4A was formulated in a slow release matrix and tested in the rat calvarial defect model. Apart from the previously reported strong stimulation of angiogenesis, fibroblast growth and collagen synthesis--the activities needed for periodontal regeneration--SBD.4A enhanced the deposition of hyaluronic acid and proliferation of osteoblasts in vitro, as well as bone regeneration in the rat calvarial defect model. Together, these results indicate the beneficial effect of SBD.4 on periodontal ligament and bone regeneration making the case for further development of this botanical growth factor.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 3T3 Cells
  • Angelica sinensis / chemistry*
  • Animals
  • Bone Regeneration / drug effects*
  • Cattle
  • Cell Differentiation
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Drugs, Chinese Herbal / pharmacology*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Hyaluronic Acid / metabolism
  • Mice
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism
  • Periodontal Diseases / drug therapy*
  • Periodontium / drug effects*
  • Periodontium / physiology
  • Phytotherapy*
  • Plant Roots / chemistry
  • Rats
  • Skull / drug effects
  • Skull / surgery


  • Drugs, Chinese Herbal
  • SBD.4A herbal preparation
  • angelicae sinensis extract
  • Hyaluronic Acid