Low-molecular-weight hyaluronan permeates through human intestinal Caco-2 cell monolayers via the paracellular pathway

Biosci Biotechnol Biochem. 2008 Apr;72(4):1111-4. doi: 10.1271/bbb.70748. Epub 2008 Apr 7.


The intestinal permeability of low-molecular-weight hyaluronan (LMW-HA) was investigated by using cultured monolayers of Caco-2 cells. The amount of LMW-HA that permeated the Caco-2 monolayers was measured by a carbazole assay. The permeability of LMW-HA increased inversely with the molecular size and was dose-dependent. The transport was observed to be energy-independent, and was correlated with the tight junction (TJ) permeability. These results suggest that LMW-HA permeated the Caco-2 cell monolayers via the paracellular pathway.

MeSH terms

  • Caco-2 Cells
  • Chromatography, High Pressure Liquid
  • Extracellular Space / metabolism*
  • Humans
  • Hyaluronic Acid / chemistry*
  • Hyaluronic Acid / metabolism*
  • Molecular Weight
  • Oligosaccharides / metabolism
  • Permeability


  • Oligosaccharides
  • Hyaluronic Acid