[Effect of Apigenin on proliferation, cell cycle and apoptosis of mouse T cells in vitro]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):337-40.
[Article in Chinese]


Aim: To study the effect of Apigenin (AP) on the proliferation, cell cycle and apoptosis of mouse T cells in vitro.

Methods: The lymphocytes were prepared from lymph nodes and thymus of mice. The effect of AP on the proliferation of T in response to ConA at different concentrations (25, 50, 100, 150, 200 micromol/L) was detected by MTT. Cell cycle was measured by PI staining and FCM. The effect of Apigenin and Apigenin with DEX on T cell apoptosis was measured by Annexin V-FITC/PI double staining and FCM. The effect of different concentrations of AP cytotoxicity to T cells was measured by MTT.

Results: 25-200 micromol/L of AP didn't have cytotoxicity to T cells, but it had some inhibitory effect on T cells in response to ConA(P<0.01), arresting cell cycle at G0/G1 in a dose-dependent manner. Different concentrations of AP inhibited the apoptosis of T cells, especially those induced by DEX(P<0.01).

Conclusion: AP can inhibit the proliferation of mouse T cells in response to ConA, arrest cell cycle at G0/G1 and inhibit the apoptosis of T cells.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Apigenin / pharmacology*
  • Apoptosis / drug effects*
  • Cell Cycle / drug effects*
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Dose-Response Relationship, Drug
  • Male
  • Mice
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects*


  • Concanavalin A
  • Apigenin