Gene expression patterns in glucose-stimulated podocytes

Biochem Biophys Res Commun. 2008 Jun 6;370(3):514-8. doi: 10.1016/j.bbrc.2008.03.121. Epub 2008 Apr 3.


To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Diabetic Nephropathies / genetics*
  • Diabetic Nephropathies / pathology
  • Disease Models, Animal
  • Gene Expression / drug effects
  • Gene Expression Profiling*
  • Glucose / metabolism*
  • Glucose / pharmacology
  • Mice
  • Podocytes / drug effects
  • Podocytes / metabolism*
  • Podocytes / pathology
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction


  • Glucose