Abstract
The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes. To harvest such transcripts cDNA libraries were produced from liver of chemical-exposed mature males. Two normalised full-length libraries were generated by different methods: (1) partial subtraction of polyA+ RNA against solid-phase cDNA using magnetic bead technology; (2) degradation of double stranded cDNA formed by abundant transcripts. To enrich for pollutant-responsive genes a subtracted EST library was also generated. For each library approximately 1.5K clones were sequenced and characterised using Blast2GO. All libraries contained pollutant-responsive transcripts not previously available while additionally the subtracted library was generally enriched approximately 1.2-10-fold for transcripts expected to be induced in response to the pollutants.
MeSH terms
-
Animals
-
Expressed Sequence Tags*
-
Gene Expression Profiling
-
Gene Expression Regulation / drug effects*
-
Gene Library*
-
Liver / drug effects*
-
Liver / metabolism*
-
Male
-
Molecular Sequence Data
-
Smegmamorpha / genetics*
-
Water Pollutants, Chemical / toxicity*
Substances
-
Water Pollutants, Chemical
Associated data
-
GENBANK/EG588214
-
GENBANK/EG588837
-
GENBANK/EG588881
-
GENBANK/EG590169
-
GENBANK/EG590172
-
GENBANK/EG590205
-
GENBANK/EG590214
-
GENBANK/EG590223
-
GENBANK/EG590252
-
GENBANK/EG590254
-
GENBANK/EG590299
-
GENBANK/EG590342
-
GENBANK/EG590361
-
GENBANK/EG590393
-
GENBANK/EG590419
-
GENBANK/EG590420
-
GENBANK/EG590432
-
GENBANK/EG590455
-
GENBANK/EG590466
-
GENBANK/EG590467
-
GENBANK/EG590468
-
GENBANK/EG590471
-
GENBANK/EG590478
-
GENBANK/EG590483
-
GENBANK/EG590546
-
GENBANK/EG590549
-
GENBANK/EG590575
-
GENBANK/EG590578
-
GENBANK/EG590588
-
GENBANK/EG590598
-
GENBANK/EG590599
-
GENBANK/EG590623
-
GENBANK/EG590659
-
GENBANK/EG590661
-
GENBANK/EG590662
-
GENBANK/EG590673
-
GENBANK/EG590674
-
GENBANK/EG590677
-
GENBANK/EG590704
-
GENBANK/EG590708
-
GENBANK/EG590737
-
GENBANK/EG590829
-
GENBANK/EG590878
-
GENBANK/EG590882
-
GENBANK/EG590888
-
GENBANK/EG590908
-
GENBANK/EG590909
-
GENBANK/EG590927
-
GENBANK/EG590934
-
GENBANK/EG590952
-
GENBANK/EG590963
-
GENBANK/EG590993
-
GENBANK/EG591098
-
GENBANK/EG591110
-
GENBANK/EG591131
-
GENBANK/EG591152
-
GENBANK/EG591178
-
GENBANK/EG591213
-
GENBANK/EG591220
-
GENBANK/EG591273
-
GENBANK/EG591274
-
GENBANK/EG591310
-
GENBANK/EG591338
-
GENBANK/EG591340
-
GENBANK/EG591387
-
GENBANK/EG591390
-
GENBANK/EG591402
-
GENBANK/EG591416
-
GENBANK/EG591437
-
GENBANK/EG591481
-
GENBANK/EG591484
-
GENBANK/EG591502
-
GENBANK/EG591549
-
GENBANK/EG591607
-
GENBANK/EG591611
-
GENBANK/EG591612
-
GENBANK/EG591685
-
GENBANK/EG591687
-
GENBANK/EG591694