Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide: effect of nitecapone

Gen Pharmacol. 1991;22(6):1063-9. doi: 10.1016/0306-3623(91)90578-t.

Abstract

1. A gastric mucosal laminin receptor has been isolated from the epithelial cell membrane by affinity chromatography on Sepharose-bound laminin, and following radioiodination was incorporated into liposomes which displayed specific affinity towards the laminin-coated surface. 2. The binding of liposomal receptor to the laminin-coated surface was inhibited by Helicobacter pylori lipopolysaccharide and reached a maximum value of 96% at 50 micrograms/ml. 3. The inhibitory effect of H. pylori lipopolysaccharide on the receptor-laminin binding was prevented by an antiulcer agent, nitecapone. The effect was concentration dependent and produced a maximum response of 83% at 30 micrograms/ml of drug concentration. 4. The results demonstrate that H. pylori is capable of disrupting gastric mucosal integrity by interfering with epithelial cell-laminin binding, and that an antiucler agent, nitecapone, counteracts this effect.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Ulcer Agents / pharmacology*
  • Catechols / pharmacology*
  • Cell Membrane / chemistry
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Chromatography, Affinity
  • Gastric Mucosa / cytology
  • Gastric Mucosa / metabolism*
  • Gastric Mucosa / pathology
  • Helicobacter pylori / metabolism*
  • In Vitro Techniques
  • Iodine Radioisotopes
  • Lipopolysaccharides*
  • Liposomes / chemistry
  • Male
  • Pentanones / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Immunologic / antagonists & inhibitors*
  • Receptors, Laminin

Substances

  • Anti-Ulcer Agents
  • Catechols
  • Iodine Radioisotopes
  • Lipopolysaccharides
  • Liposomes
  • Pentanones
  • Receptors, Immunologic
  • Receptors, Laminin
  • nitecapone