Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans

Mech Ageing Dev. 2008 Jun;129(6):322-31. doi: 10.1016/j.mad.2008.02.011. Epub 2008 Mar 2.


We have shown that platinum nanoparticles (nano-Pt) are a superoxide dismutase (SOD)/catalase mimetic. Various data have shown extension of the Caenorhabditis elegans lifespan by antioxidant treatment. The present study was designed to elucidate the survival benefit conferred by nano-Pt, as compared to the well-known SOD/catalase mimetic EUK-8. At 0.5mM, nano-Pt significantly extended the lifespan of wild-type N2 nematodes and at 0.25 and 0.5mM, nano-Pt recovered the shortened lifespan of the mev-1(kn1) mutant, which is due to excessive oxidative stress. In both instances, EUK-8 at 0.05, 0.5, and 5mM did not extend nematode lifespan. Even when 0.4M paraquat was loaded exogenously, nano-Pt (0.1 and 0.5mM) and EUK-8 (0.5 and 5mM) were effective in rescuing worms. Moreover, 0.5mM nano-Pt significantly reduced the accumulation of lipofuscin and ROS induced by paraquat. We measured the in vitro dose-dependent quenching of O(2)(-) and H(2)O(2), indicating that nano-Pt is a more potent SOD/catalase mimetic than EUK-8. Nano-Pt prolonged the worm lifespan, regardless of thermotolerance or dietary restriction. Taken together, nano-Pt has interesting anti-ageing properties.

MeSH terms

  • Animal Feed
  • Animals
  • Antioxidants / pharmacology*
  • Caenorhabditis elegans
  • Catalase / metabolism
  • Dose-Response Relationship, Drug
  • Hydrogen Peroxide / metabolism
  • Lipofuscin / metabolism
  • Longevity / drug effects*
  • Metal Nanoparticles*
  • Microscopy, Fluorescence
  • Oxidative Stress
  • Platinum / pharmacology*
  • Superoxide Dismutase / metabolism
  • Time Factors


  • Antioxidants
  • Lipofuscin
  • Platinum
  • Hydrogen Peroxide
  • Catalase
  • Superoxide Dismutase