A significant increase in intracellular Ca2+ concentration induced by (2S,3R,4S)-2-(carboxycyclopropyl)glycine, a new potent NMDA agonist, in cultured rat hippocampal neurons

Brain Res. 1991 Dec 20;567(2):342-5. doi: 10.1016/0006-8993(91)90817-f.

Abstract

The increase in intracellular Ca2+ concentration, [Ca2+]i, induced by isomers of 2-(carboxycyclopropyl)glycine (CCG) was examined in cultured rat hippocampal neurons. Some CCG isomers and N-methyl-D-aspartate (NMDA) increased [Ca2+]i in a concentration dependent manner. The 2S,3R,4S isomer of CCG (L-CCG-IV) was the most potent in elevating [Ca2+]i, and its activity was more than 100 times higher than that of NMDA and about 10 times higher than that of L-glutamate. The increase in [Ca2+]i was effectively blocked by NMDA blockers and Mg2+, and was markedly augmented by the addition of a low concentration of glycine. L-CCG-IV would be a useful tool for elucidation of functions of NMDA receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids, Dicarboxylic / antagonists & inhibitors
  • Amino Acids, Dicarboxylic / pharmacology*
  • Animals
  • Calcium / metabolism*
  • Cells, Cultured
  • Hippocampus / cytology
  • Hippocampus / metabolism*
  • N-Methylaspartate / pharmacology*
  • Neurons / drug effects
  • Neurons / metabolism*
  • Rats
  • Receptors, N-Methyl-D-Aspartate / drug effects*

Substances

  • Amino Acids, Dicarboxylic
  • Receptors, N-Methyl-D-Aspartate
  • (alpha-carboxycyclopropyl)glycine
  • N-Methylaspartate
  • Calcium