Abstract
An ethanol extract of Helichrysum maracandicum showed antiproliferative activity against cultured cells of SENCAR mouse in an in vitro assay, and activity-guided fractionation of the extract resulted in the isolation of isosalipurposide as an active substance. Naringenin chalcone, the aglycone of isosalipurposide, also showed strong antiproliferative activity. An in vivo assay of two-stage carcinogenesis on mouse skin revealed that epidermal application of isosalipurposide resulted in delayed formation of papillomas. Western blot analysis showed that the expression of p38 mitogen-activated protein kinase was suppressed by the administration of naringenin chalcone or isosalipurposide, which might be related to the anticarcinogenic activity.
MeSH terms
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9,10-Dimethyl-1,2-benzanthracene
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Animals
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Anticarcinogenic Agents / chemistry
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Anticarcinogenic Agents / isolation & purification*
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Blotting, Western
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Cell Line, Transformed
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Cell Line, Tumor
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Chalcones / chemistry
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Chalcones / isolation & purification*
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Cocarcinogenesis
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Female
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Flowers / chemistry
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Helichrysum / chemistry*
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Mice
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Mice, Inbred SENCAR
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Papilloma / chemically induced
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Papilloma / enzymology
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Papilloma / prevention & control
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Phorbol Esters
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Plant Extracts / chemistry
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Plants, Medicinal
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Skin Neoplasms / chemically induced
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Skin Neoplasms / enzymology
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Skin Neoplasms / prevention & control
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Uzbekistan
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Anticarcinogenic Agents
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Chalcones
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Phorbol Esters
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Plant Extracts
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isosalipurposide
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9,10-Dimethyl-1,2-benzanthracene
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naringenin chalcone
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p38 Mitogen-Activated Protein Kinases