Glucose fluxes during OGTT in adolescents assessed by a stable isotope triple tracer method

J Pediatr Endocrinol Metab. 2008 Jan;21(1):31-45. doi: 10.1515/jpem.2008.21.1.31.


Virtually no information is available on glucose fluxes during a meal or glucose ingestion in adolescents.

Aim: To use a triple tracer approach to measure rates of appearance of ingested glucose (Ra(ogtt)), endogenous glucose production (EGP) and glucose disappearance (Rd) following an oral glucose bolus in adolescents.

Methods: Eleven adolescents (4 M/7 F, 15 +/- 1 yr; 67.3 +/- 4.7 kg; 24 +/-2 kg/m2) underwent a frequent sampled oral glucose tolerance test (OGTT) (labelled with [6,6-2H2]glucose) combined with intravenous infusion of [1-(13)C]glucose and [U-(13)C6]glucose following an overnight fast. Formulas were developed to estimate glucose fluxes using one- or two-compartment models.

Results: During the 7 h following the OGTT bolus, 9.8 +/- 2.3% of the ingested glucose was extracted by the liver, EGP was suppressed by 45 +/- 4% and Rd increased by 21 +/- 5%.

Conclusions: The triple tracer method provided accurate assessment of Ra(ogtt), EGP and Rd fluxes during an OGTT in adolescents. Thus, this method might provide novel insight on postprandial glucose fluxes in children/adolescents under various conditions.

Publication types

  • Clinical Trial

MeSH terms

  • Adolescent
  • Aging / metabolism
  • Blood Glucose / metabolism*
  • Carbon Isotopes
  • Deuterium
  • Diabetes Mellitus / blood
  • Diabetes Mellitus / diagnosis*
  • Female
  • Glucose / metabolism
  • Glucose Tolerance Test / methods*
  • Humans
  • Male
  • Models, Biological
  • Radioactive Tracers*
  • Tritium


  • Blood Glucose
  • Carbon Isotopes
  • Radioactive Tracers
  • Tritium
  • Deuterium
  • Glucose