Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation

Cell. 1991 May 3;65(3):395-407. doi: 10.1016/0092-8674(91)90457-a.


Myn, a novel murine approximately 18 kd basic/helix-loop-helix/"leucine zipper" (B/HLH/LZ) protein, forms a specific DNA-binding complex with the c-Myc oncoprotein through the HLH/LZ motif in both proteins. c-Myc/Myn recognizes a c-Myc-binding site (GACCACGTGGTC) with higher affinity than either protein by itself. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the c-Myc/Myn complex in vivo. In 3T3 fibroblasts, Myn mRNA levels are induced several-fold by serum with delayed early kinetics, suggesting regulation by immediate-early gene products. Coexpression of Myn in a myc/ras rat embryo fibroblast focus formation assay specifically augmented c-myc transforming activity. We suggest that interaction of Myn with c-Myc stabilizes sequence-specific DNA binding in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Blood
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • DNA / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation
  • Genes, myc*
  • Genes, ras*
  • Leucine Zippers*
  • Methylation
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Transfection


  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Proto-Oncogene Proteins c-myc
  • Max protein, mouse
  • DNA

Associated data

  • GENBANK/M63903
  • GENBANK/M64376
  • GENBANK/M64377
  • GENBANK/M64378
  • GENBANK/M64379
  • GENBANK/M64380
  • GENBANK/M64381
  • GENBANK/M64382
  • GENBANK/M64383
  • GENBANK/M64384