Gamma-tubulin is a highly conserved component of the centrosome

Cell. 1991 May 31;65(5):825-36. doi: 10.1016/0092-8674(91)90390-k.

Abstract

We have cloned and characterized gamma-tubulin genes from both X. laevis and S. pombe, and partial genes from maize, diatom, and a budding yeast. The proteins encoded by these genes are very similar to each other and to the original Aspergillus protein, indicating that gamma-tubulins are an ubiquitous and highly conserved subfamily of the tubulin family. A null mutation of the S. pombe gene is lethal. gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. In particular, gamma-tubulin is associated with the pericentriolar material, the microtubule-nucleating material of the centrosome. gamma-Tubulin remains associated with the centrosome when microtubules are depolymerized, suggesting that it is an integral component that might play a role in microtubule organization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aspergillus / genetics
  • Base Sequence
  • Biological Evolution
  • Cell Line
  • Cytoskeleton / physiology*
  • Cytoskeleton / ultrastructure
  • Fluorescent Antibody Technique
  • Genes
  • Genes, Fungal
  • Microtubules / physiology*
  • Microtubules / ultrastructure
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Schizosaccharomyces / genetics*
  • Sequence Homology, Nucleic Acid
  • Tubulin / analysis
  • Tubulin / genetics*
  • Xenopus laevis

Substances

  • Oligonucleotide Probes
  • Tubulin

Associated data

  • GENBANK/M60958
  • GENBANK/M60959
  • GENBANK/M60960
  • GENBANK/M60961
  • GENBANK/M60962
  • GENBANK/M63446
  • GENBANK/M63447
  • GENBANK/M64383
  • GENBANK/M64384
  • GENBANK/M86183