The Drosophila Dorsal-Ventral Patterning Gene Tolloid Is Related to Human Bone Morphogenetic Protein 1

Cell. 1991 Nov 1;67(3):469-81. doi: 10.1016/0092-8674(91)90522-z.

Abstract

Mutations in the Drosophila tolloid (tld) gene lead to a partial transformation of dorsal ectoderm into ventral ectoderm. The null phenotype of tld is similar to, but less severe than decapentaplegic (dpp), a TGF-beta family member required for the formation of all dorsal structures. We have cloned the tld locus by P element tagging. At the blastoderm stage, tld RNA is expressed dorsally, similar to that described for dpp. Analysis of a tld cDNA reveals three sequence motifs: an N terminal region of similarity to a metalloprotease, two EGF-like repeats, and five copies of a repeat found in human complement proteins C1r and C1s. tld sequence is 41% identical to human bone morphogenetic protein 1 (BMP-1); the closest members to dpp within the TGF-beta superfamily are BMP-2 and BMP-4, two other bone morphogenetic proteins. These findings suggest that these genes are members of a signal generating pathway that has been conserved between insects and mammals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bone Morphogenetic Proteins
  • Cloning, Molecular
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Gene Expression
  • Genes*
  • Insect Hormones / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • Morphogenesis*
  • Nucleic Acid Hybridization
  • Protein Biosynthesis
  • Proteins / genetics*
  • RNA, Messenger / genetics
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Tolloid-Like Metalloproteinases

Substances

  • Bone Morphogenetic Proteins
  • Drosophila Proteins
  • Insect Hormones
  • Proteins
  • RNA, Messenger
  • Tolloid-Like Metalloproteinases
  • tld protein, Drosophila

Associated data

  • GENBANK/M76976
  • GENBANK/S60911
  • GENBANK/S60914
  • GENBANK/S60917
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S60969
  • GENBANK/S61522
  • GENBANK/S61524
  • GENBANK/S63567