The M-twist gene of Mus is expressed in subsets of mesodermal cells and is closely related to the Xenopus X-twi and the Drosophila twist genes

Dev Biol. 1991 Feb;143(2):363-73. doi: 10.1016/0012-1606(91)90086-i.

Abstract

The twist gene was characterized in Drosophila as being necessary at gastrulation for the establishment of the mesodermal germ layer. It codes for a nuclear DNA-binding protein that is probably a transcription factor. We have cloned and sequenced the M-twist gene of Mus musculus. The deduced proteins encoded by the Mus, Xenopus, and Drosophila twist cDNAs, respectively, show a high degree of similarity. Northern blot analyses and in situ hybridizations reveal that the 1.7-kb murine M-twist m-RNA is present at early stages, starting at 8 days post coitum, and is expressed the most at 9.5 days in the cephalic and branchial mesectoderm, in some derivatives of the mesodermal layer (sclerotoma and somatopleura), and in the limb buds.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / genetics*
  • Drosophila melanogaster / genetics
  • Gene Expression
  • Mice / embryology*
  • Mice / genetics*
  • Myogenic Regulatory Factors*
  • Nuclear Proteins / genetics*
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • Transcription, Genetic
  • Twist-Related Protein 1
  • Xenopus laevis / genetics

Substances

  • DNA-Binding Proteins
  • Myogenic Regulatory Factors
  • Nuclear Proteins
  • RNA, Messenger
  • Twist-Related Protein 1
  • Twist1 protein, mouse
  • DNA

Associated data

  • GENBANK/M55056
  • GENBANK/M55058
  • GENBANK/M63649
  • GENBANK/M63650
  • GENBANK/M65223
  • GENBANK/M65224
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S89458
  • GENBANK/X59082