Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives

Gene. 1991 Jun 15;102(1):75-8. doi: 10.1016/0378-1119(91)90541-i.


A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chloramphenicol Resistance / genetics
  • Genetic Vectors / physiology*
  • Kanamycin Resistance / genetics
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Promoter Regions, Genetic / genetics
  • beta-Galactosidase / genetics


  • beta-Galactosidase

Associated data

  • GENBANK/D90323
  • GENBANK/D90324
  • GENBANK/D90325
  • GENBANK/D90326
  • GENBANK/D90327
  • GENBANK/S49891
  • GENBANK/X53937
  • GENBANK/X53938
  • GENBANK/X53939
  • GENBANK/X55890