Wee1(+)-like gene in human cells

Nature. 1991 Sep 5;353(6339):80-3. doi: 10.1038/353080a0.


The wee1+ gene is a mitotic inhibitor controlling the G2 to M transition of the fission yeast Schizosaccharomyces pombe and encodes a protein kinase with both serine- and tyrosine-phosphorylating activities. We have cloned a human gene (WEE1Hu) similar to wee1+ by transcomplementation of a yeast mutant. WEE1Hu encodes a protein homologous to the S. pombe wee1+ and mik1+ (a functionally redundant sibling of wee1+) kinases and effectively rescues a wee1 mutation. We report here that overexpression of WEE1Hu in fission yeast generates very elongated cells as a result of inhibition of the G2-M transition in the cell cycle. In addition, we detected a 3-kilobase-long WEE1Hu messenger RNA in all the human cell lines we examined. We conclude that a wee1(+)-like gene exists and is expressed in human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Gene Expression
  • Humans
  • Mitosis / genetics
  • Molecular Sequence Data
  • Protein Kinases / genetics*
  • RNA, Messenger / analysis
  • Restriction Mapping
  • Schizosaccharomyces / genetics
  • Sequence Homology, Nucleic Acid
  • Transfection


  • RNA, Messenger
  • Protein Kinases

Associated data

  • GENBANK/X57450
  • GENBANK/X57451
  • GENBANK/X57452
  • GENBANK/X57453
  • GENBANK/X57454
  • GENBANK/X57455
  • GENBANK/X57689
  • GENBANK/X62048
  • GENBANK/X62741
  • GENBANK/X62744